Free-radical first responders: The characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, <em>Rana sylvatica</em>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Free-radical first responders: The characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, Rana sylvatica

Authors:	Neal J Dawson Barbara A KatzenbackKenneth B Storey

Institution:	Department of Biology and Institute of Biochemistry Carleton University, Ottawa, ON, Canada

Abstract:

Keywords:	ALS amyotrophic lateral sclerosis AMPK 5&prime adenosine monophosphate-activated protein kinase C_m the denaturant concentration at which half of the protein is unfolded CuZnSOD copper and zinc containing SOD DEAE⁺ diethylaminoethyl cellulose DEPC diethylpyrocarbonate DTT dithiothreitol FeSOD iron containing superoxide dismutase K_m Michaelis&ndash Menten constant IMAC immobilized metal ion affinity chromatography IP₃ inositol trisphosphate KPi potassium phosphate MAPK mitogen activated protein kinase MnSOD manganese containing superoxide dismutase NBT nitrotetrazolium blue NiSOD nickel containing superoxide dismutase PKA protein kinase A PKC protein kinase C PKG protein kinase G PMSF phenylmethylsulfonyl fluoride PP1 protein phosphatase 1 PP2A protein phosphatase 2A PP2C protein phosphatase 2C PVDF polyvinylidene difluoride ROS reactive oxygen species TBST Tris buffered saline with Tween V_max maximal velocity
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