Characterization of receptor for granulocyte colony-stimulating factor on human circulating neutrophils |
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| Authors: | H Uzumaki T Okabe N Sasaki K Hagiwara F Takaku S Itoh |
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| Institution: | Third Department of Internal Medicine, Faculty of Medicine, University of Tokyo, Japan. |
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| Abstract: | We have made a mutein of human G-CSF with more stable, and potent biological activity. Using 125 I-labeled mutein human G-CSF, high affinity binding sites were identified on human circulating neutrophils. Receptor number per cell was 560 with a Kd of 250 pM. The human G-CSF receptor was identified as a single subunit protein of Mr approximately 150,000. |
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