Cloning and nucleotide sequence analysis of the serotype 2 fimbrial subunit gene of Bordetella pertussis |
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Authors: | I. Livey C.J. Duggleby A. Robinson |
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Affiliation: | Molecular Genetics Laboratory, PHLS, Centre for Applied Microbiology and Research, Salisbury, Wiltshire, UK. |
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Abstract: | An oligonucleotide probe complementary to the beginning of the gene encoding the serotype 2(ST2) fimbrial subunit of Bordetella pertussis was synthesized and a cloned DNA fragment hybridizing with the probe identified and sequenced. Several lines of evidence indicate that an open reading frame with coding information for a polypeptide of 207 amino acids, including a 26-amino-acid signal sequence, is the ST2 gene. The protein deduced from the nucleotide sequence shows good agreement with the NH2-terminal amino acid sequence, amino acid composition and molecular weight of the purified fimbrial subunit. In addition, the proposed ST2 subunit is shown to have homology with other fimbrial subunits. |
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