| 快速提取、纯化叶绿体4.5SrRNA的方法 |
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| 引用本文： | 谷丽雅,肖啸,王鄂生,程振起. 快速提取、纯化叶绿体4.5SrRNA的方法[J]. 中国生物化学与分子生物学报, 1985, 1(Z1): 97-100 |
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| 作者姓名： | 谷丽雅 肖啸 王鄂生 程振起 |
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| 作者单位： | 武汉大学生物系(谷丽雅，肖啸，王鄂生)，中科院北京生物物理所(程振起) |
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| 摘 要： | 我们采用植物叶与热缓冲液、苯酚直接混合(约65℃)匀浆,离心抽提和乙醇沉淀后,得到植物叶总RNA。经聚丙烯酰胺凝胶电泳分离、纯化,即可得到叶绿体4.5S rRNA,此法不仅操作简单,而且得率高。 同时,经过对同一植物的不同组织或不同细胞组分,如根、细胞质、叶绿体和叶绿体核糖体小分子RNA的提取与鉴定,以简便的方法证明了4.5S rRNA是叶绿体核糖体成份,也证明了我们所采用的提取、纯化4.5SrRNA方法的可靠性。
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| 关 键 词： | 叶绿体 小分子DNA 纯化 聚丙烯酰胺凝胶电泳 |
| 收稿时间： | 1985-12-20 |
RAPID ISOLATION AND PURIFICATION OF CHLOROPLAST 4.5S RIBOSOMAL RNA |
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| Gu,Li-ya Xiao,Xiao Wang,E-sheng. RAPID ISOLATION AND PURIFICATION OF CHLOROPLAST 4.5S RIBOSOMAL RNA[J]. Chinese Journal of Biochemistry and Molecular Biology, 1985, 1(Z1): 97-100 |
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| Authors: | Gu Li-ya Xiao Xiao Wang E-sheng |
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| Affiliation: | (Department of Biology, Wuhan University, Wuhan.)Cheng, Zhen-qi (Institute of Biophysics, Academia Sinica, Beijing. |
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| Abstract: | A rapid, simple method for purification of chloroplast 4.5S rRNA was described. Total RNAs were ext-racted directly from whole green leaves and 4.5S rRNA was purified by electrophoresis on denatured gel. The purified 4.5S rRNA appeared as a single band on the polyacrylamide gel and had a typical UV absorption peak of nucleic acids. To prove that this RNA was a component of chloroplasts, RNAs from roots, cytoplasm, chloroplasts and chloroplast ribosomes were prepared and analyzed by gel electrophoresis. The results showed that the 4.5S rRNA was a component of chloroplast ribosomes and the method of purification is reliable. |
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| Keywords: | chloroplast Low-molecular weight RNA purification polyacrylamide gel electrophoresis. |
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