武汉地区克氏原螯虾繁殖期的研究

为探究武汉地区克氏原螯虾(Procambarus clarkii)繁殖期的问题,采用石蜡切片的方法,对武汉地区自然群体中克氏原螯虾雌雄成体的生殖腺进行了周年观察研究。结果表明,武汉地区成年克氏原螯虾的卵母细胞发育不同步,在4~5月以及9~10月的卵巢中都有大量成熟期的卵子,同时也含有少量卵黄发生前期或初期的卵母细胞。6~7月和11月都可见产后恢复期的卵巢,内含少量未产出的正在退化的过熟卵和一些卵黄发生前期或初期的卵母细胞。精巢发育的年周期变化没有卵巢变化的那么明显,但也可以看到,在4~6月和9~10月,含精子细胞和精子的精小管的比例大大增加,而在其他时期,含精原细胞和精母细胞的精小管比例较多。这些结果提示,武汉地区克氏原螯虾一年有两个繁殖高峰期,一个在4~5月,另一个在9~10月。

Reproductive Period of Procambarus clarkii in Wuhan Area

The reproductive period of Procambarus clarkii in Wuhan area was investigated to provide basic data for developing artificial reproduction technique.The asynchronism of oogenesis was observed in the sections of ovaries.The mature eggs appeared in the sections of the ovaries sampled in two periods.One period was from April to May,and the other one was from September to October.The large eggs contained abundant yolk granules.From June to July and in November,more previtellogenic and initial vitellogenic oocytes were observed in the ovaries,although there were a few eggs in degeneration,indicating that these periods might oogenesis recovery periods after spawning.In crayfish testis,the proportion of the testicular tubules containing spermatids and sperm increased dramatically in two periods,April to June and September to October,although spermatocytes were also observed during these two periods.Furthermore,the vas deferens containing spermatozoa were frequently observed in these two periods.The ratio of the testicular tubules containing the spermatogonia and spermatocytes was higher in other periods.These results suggest that P.clarkii have two periods of reproductive peak in Wuhan area,from April to May and from September to October.