Metabolic fluxes during the growth of thermotolerant methylotrophic Bacillus strains in methanol-sufficient chemostat cultures |
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Authors: | Anthony G. Brooke Margaret M. Attwood David W. Tempest |
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Affiliation: | (1) Department of Microbiology, University of Sheffield, S10 2TN Sheffield, UK;(2) Present address: ABM-Sturge Division, RTZ Chemicals, Denison Road, Y08 8EF Selby, North Yorkshire, UK |
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Abstract: | Growth of the thermotolerant methylotrophic Bacillus strain TS1 in methanol-limited chemostat culture showed that the substrate was oxidized solely to biomass and CO2. When a pulse of methanol was added to the growth vessel anabolism could be shown to be dissociated from catabolism for a transient period of time. Present data shows that when the organism was grown with a limitation other than carbon, some of the substrate was channelled into metabolite over-production. When the organism was grown under N-limitation 2-oxoglutarate accumulated in the culture medium in small amounts whilst acetate accumulated under all carbon excess conditions. Although the average carbon recovery was 92%, analysis of the culture filtrates for other metabolites failed to show significant amounts of any individual product above those detected in carbon-limited growth comditions. The results are discussed in relation to published data. |
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Keywords: | Methylotrophy Thermotolerance Chemostat culture Methanol excess Metabolic fluxes Overflow metabolites |
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