胀果甘草悬浮培养细胞合成甘草总黄酮

比较了胀果甘草(Glycyrrhiza inflata)悬浮细胞在逐级放大摇瓶中的生长、黄酮产量以及营养消耗过程,以便了解其放大规律。结果表明,在250和500mL摇瓶中,细胞的最大生物量、黄酮产量以及最大比生长速率没有显著性差异,但是在1L的摇瓶中,这三种参数都显著地降低,分别比250mL摇瓶中降低了27%,30%和27%。在逐级放大的摇瓶中,氮、磷、铵浓度都随着培养时间延长而逐渐降低,尽管在1L的摇瓶中磷消耗得最慢,但三种摇瓶中磷在细胞生长对数期基本都被消耗尽了。此外,硝态氮在第18天时基本被消耗完,而铵态氮在细胞收获时仍能维持在100mg/L。因此在反应器中培养时,主要的培养条件还需进一步优化。

Production of Flavonoids in Cell Suspension Culture of Glycyrrhiza inflata (Leguminosae)

The cell biomass,flavonoids production and nutrients consumption were monitored to characterize the culture of progressive scale-up of Glycyrrhiza inflata.The maximum biomass,flavonoids production and the maximum specific growth rate in the culture of 250 and 500 mL flasks remain similar,but were significantly higher than that of 1 000 mL flask.The three parameters in the culture of 1 000 mL flask were 27%,30%,and 27% lower than that of 250 mL flask,respectively.The concentrates of phosphate,nitrate and ammonium in progressive scale-up flasks decreased similarly.Phosphate and nitrate were almost exhausted on day 10 and 18 in all flasks,respectively;while ammonium maintained about 100 mg/L till cells were harvested.The basic culture conditions needed to be further optimized for higher flavonoids production on a bioreactor scale.