Construction of a broad-host-range non-mobilizable stable vector carrying RP4 par-region. |
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| Authors: | J Crouzet S Lévy-Schil L Cauchois B Cameron |
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| Affiliation: | Département Biotechnologies, Rh?ne-Poulenc Rorer S.A., Vitry sur Seine, France. |
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| Abstract: | Plasmid pXL1635 was constructed from the already segregationally stable incP-derived pRK290. Plasmid pXL1635 should be suitable for industrial and environmental uses in Gram- bacteria since (i) it contains the par fragment from RP4 which increases its stability in Pseudomonas denitrificans, a cobalamin-producing and industrially used bacterium, and (ii) the RK2 oriT has been deleted, leading to a non-mobilizable plasmid. |
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