ATP increases chemoattractant induced cyclic GMP accumulation in Dictyostelium discoideum |
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Authors: | Jose M. Mato |
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Affiliation: | Laboratory of Zoology, Cell Biology and Morphogenesis Unit, Kaiserstraat 63, University of Leiden, Leiden, The Netherlands |
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Abstract: | Changes in guanosine cyclic 3′,5′-monophosphate associated with adenosine cyclic 3′,5′-monophosphate and folic acid addition in the presence of ATP have been examined in Dictyostelium discoideum. Preincubation with 1 mM ATP had no effect on the basal cyclic GMP level but increased the cycli GMP accumulation in response to cylci AMP (5·10−8 M) or folic acid (5·10−6 M) 40–50%. ATP could not be replaced by ADP of 5′-adenylyliminodiphosphate. Because ATP has no effect on cyclic AMP receptor binding these results indicate that structural membrane alterations (e.g. membrane phosphorylation) may control the transduction of a chemotactic signal. |
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Keywords: | App(NH)p 5′-adenylyliminophosphate AMPMe 5′-adenosine monophosphate methyl ester |
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