| 人乳铁蛋白cDNA的克隆及序列分析 |
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| 引用本文： | 程华 陈秀珠 还连栋. 人乳铁蛋白cDNA的克隆及序列分析[J]. 生物工程学报, 2001, 17(4): 385-387 |
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| 作者姓名： | 程华 陈秀珠 还连栋 |
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| 作者单位： | 中国科学院微生物研究所,北京,100080 |
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| 基金项目： | 中国科学院"九五"重点项目(KY95-J1-331). |
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| 摘 要： | 从北京正常人乳腺组织中提取总RNA,用RT-PCR的方法扩增人乳铁蛋白(hLF)的cDNA,将其克隆到pGEM-T载体上并进行DNA序列测定。结果表明,所克隆的hLF cDNA序列全长为2136bp,其DNA序列与GenBank中另外5个hLF cDNA序列相比,有2个碱基与这5个序列不同：1740位这5个序列是G,本文序列是C;1756位这5个序列是T,本文序列是C。其中1740位碱基的变化导致了第580位氨基酸由Glu变为Asp。
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| 关 键 词： | 人乳铁蛋白 cDNA RTPCR 基因克隆 DNA序列分析 |
| 文章编号： | 1000-3061(2001)04-0385-03 |
| 修稿时间： | 2000-12-31 |
cDNA Cloning and Sequence Analysis of Human Lactoferrin |
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| H Cheng,X Z Chen,L D Huan. cDNA Cloning and Sequence Analysis of Human Lactoferrin[J]. Chinese journal of biotechnology, 2001, 17(4): 385-387 |
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| Authors: | H Cheng X Z Chen L D Huan |
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| Affiliation: | Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, China. |
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| Abstract: | Human lactoferrin (hLF) cDNA was amplified by RT-PCR from normal human mammary tissue obtained from Daxing County of Beijing City of China, and then subcloned into pGEM-T vector. hLF cDNA sequence was determined, which consists of 2136 bp. Comparison with five other hLF cDNA sequences registered in GenBank shows 99% homology in DNA sequence. However, there are two base substitutions (nucleotide 1740 G-->C, nucleotide 1756 T-->C), one of which subsequently leads to an amino acid change (residue 580 Glu-->Asp). |
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| Keywords: | Human lactoferrin cDNA RT-PCR Gene cloning DNA sequence analysis |
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