Preparation of myosin rods from myofibrils |
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Authors: | C Oriol M F Landon N van Thoai |
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Affiliation: | Laboratoire de Biochimie Générale et Comparée, Collège de France, 75231 Paris Cedex 05, France |
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Abstract: | A simple and fast procedure is described for producing myosin rods with a good yield. The method is based on the digestion of myofibrils by soluble papain. After purification by alcoholic precipitation and gel filtration, rod segments exhibited physical-chemical parameters identical to previously reported data. |
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