1H, 13C, and 15N chemical shifts assignments for human endothelial monocyte-activating polypeptide EMAP II |
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Authors: | Dmytro Lozhko Jan Stanek Krzysztof Kazimierczuk Anna Zawadzka-Kazimierczuk Wiktor Kozminski Igor Zhukov Alexander Kornelyuk |
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Institution: | 1. Institute Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Acad. Zabolotnogo 150, 03-143, Kyiv, Ukraine 2. Faculty of Chemistry, University of Warsaw, Pasteura 1, 02-093, Warsaw, Poland 3. Slovenian NMR Centre, National Institute of Chemistry, Hajdrihova 19, 1001, Ljubljana, Slovenia 4. EN-FIST Center of Excellence, Dunajska 156, 1000, Ljubljana, Slovenia 5. Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5a, 02-106, Warsaw, Poland
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Abstract: | Endothelial and monocyte-activating polypeptide II (EMAP II) is a cytokine that plays an important role in inflammation, apoptosis and angiogenesis processes in tumour tissues. Structurally, the EMAP II is a 169 amino acid residues long C-terminal domain (residues 147–312) of auxiliary tRNA binding protein p43. In spite of existence in pdb databank of two X-ray structures there are some important aspects of EMAP II cytokine function which are still not fully understood in detail. To obtain information about 3D structure and backbone dynamic processes in solution we perform structure evaluation of human EMAP II cytokine by NMR spectroscopy. The standard approach to sequence-specific backbone assignment using 3D NMR data sets was not successful in our studies and was supplemented by recently developed 4D NMR experiments with random sampling of evolution time space. Here we report the backbone and side chain 1H, 13C, and 15N chemical shifts in solution for recombinant EMAP II cytokine together with secondary structure provided by TALOS + software. |
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