Isolation and characterization of a novel soil strain, Pseudomonas cepacia BY21, with glutaryl-7-aminocephalosporanic acid acylase activity |
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Authors: | Yong-Ho Khang Byong-Hoon Yoo |
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Institution: | (1) Department of Applied Microbiology, Yeungnam University, Kyongsan, 712-749, Republic of Korea |
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Abstract: | A search was undertaken to screen microorganisms in soil which produce an enzyme capable of deacylating glutaryl-7-aminocephalosporanic acid (glutaryl-7-ACA) to 7-aminocephalosporanic acid (7-ACA). To facilitate screening, a model substrate, glutaryl-p-nitroanilide, and a 7-ACA sensitive strain, Enterobacter taylorae BY312, were used as a color indicator and bioassay, respectively. An isolate, Pseudomonas cepacia BY21, was found to produce glutaryl-7-ACA acylase, of which the activity was optimal at pH 8.0 and 45 °C. |
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Keywords: | 7-aminocephalosporanic acid bioconversion cephalosporin C glutaryl-7-ACA acylase |
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