Aquaporin functionality in relation to H+-ATPase activity in root cells of Capsicum annuum grown under salinity |
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Authors: | Martínez-Ballesta M. Carmen Martínez Vicente Carvajal Micaela |
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Affiliation: | Departamento de Nutrición y Fisiología Vegetal, Centro de Edafología y Biología Aplicada del Segura, CSIC, PO Box 4195, 30080 Murcia, Spain |
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Abstract: | As water and nutrient uptake should be related in the response of plants to salinity, the aim of this paper is to establish whether or not aquaporin functionality is related to H+-ATPase activity in root cells of pepper ( Capsicum annuum L.) plants. Thus, H+-ATPase activity was measured in plasma membrane vesicles isolated from roots and aquaporin functionality was measured using a cell pressure probe in intact roots. Salinity was applied as 60 m M NaCl or 60 m M KCl, to determine which ion (Na+, K+ or Cl−) is producing the effects. We also investigated whether the effects of both salts were ameliorated by Ca2+. Similar results were obtained for cell hydraulic conductivity, Lpc, and H+-ATPase activity, large reductions in the presence at NaCl or KCl and an ameliorative effect of Ca2+. However, fusicoccin (an activator of H+-ATPase) did not alter osmotic water permeability of protoplasts isolated from roots. Addition of Hg2+ inhibited both ATPase and aquaporins, but ATPase also contains Hg-binding sites. Therefore, the results indicate that H+-ATPase and aquaporin activities may not be related in pepper plants. |
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