|
|||||
|
|
Transformation of Penicillium chysogenum with selection for increased resistance to benomyl |
|
| Authors: | Tessa M. Picknett Gunter Saunders |
| Affiliation: | Centre for Biotechnology, Imperial College of Science Technology and Medicine, School of Biotechnology, London, U.K.;Polytechnic of Central London, School of Biotechnology, London, U.K. |
| Abstract: | Abstract Incubation of protoplasts of Penicillium chrysogenum with the plasmid pBT-3 permitted selection of transformants on the basis of increased resistance to the anti-fungal agent benomyl. Transformants were obtained at a frequency of 1–2 per μg of DNA. Southern analysis revealed that transformation had occured by integration of vector sequences into the host genome. |
| Keywords: | Penicillium chrysogenum Benomyl Transformation |