DNA degradation and reduced recombination following UV irradiation during meiosis in yeast (Saccharomyces cerevisiae) |
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Authors: | Yehiam Salts Giora Simchen and Ramon Pi?on |
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Institution: | (1) Department of Biology, University of California, San Diego, 92093 La Jolla, California;(2) Department of Genetics, The Hebrew University of Jerusalem, Jerusalem, Israel |
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Abstract: | Summary Irradiation of meiotic yeast cells with moderate doses of ultraviolet irradiation (1,600 erg/mm2) leads to the arrest of premeiotic DNA synthesis, massive (5–40%) DNA degradation, and a 40–50% loss of cell viability. In contrast, such doses of UV irradiation had a minor effect on viability (15–20% loss) of logarithmically growing cells, and no comparable DNA degradation was observed in irradiated synchronized vegetative cells. Meiotic recombination is also affected by UV irradiation. When administered at a stage comparable to meiotic prophase, low doses of irradiation result in a reduction in recombination frequency without significantly affecting cell viability. |
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