Production and Accumulation of Xylooligosaccharides with Long Chains by Growing Culture and Xylanase of a Mutant Strain of Bacillus pumilus X-6-19 |
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Authors: | Qingzhu Yuan Tsuyoshi Adachi Shinji Takenak Shuichiro Murakami Machiko Tanaka Kenji Aoki |
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Affiliation: | 1. Division of Life Science, Graduate School of Science and Technology, Kobe University, Rokko, Kobe 657-8501, Japan 2. Department of Applied Biological Chemistry, Graduate school of Agricultural Science, Kobe University, Rokko, Kobe 657-8501, Japan 3. Research & Development Deparment, Bunsen Co.,Ltd., Shingu-cho,Tatsuno 679-4393, Japan |
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Abstract: | Bacillus pumilus X-6-9 isolated from soil and subsequently identified, produced xylooligosacchatides with long chainsfrom xylan and accumulated them in the culture. By improving the culture conditions and mutating the bacterium, a 3.2-fold increasein the production of the xylooligosaccharides was established, when compared to the original culture conditions of B. pumilus X-6-19.The addition of D-glucose to the culture of the mutant swain U-3 of B. pumilus X-6-9 repressed the synthesis of β-xylosidase, but notxylanase. Thus, it was revealed that strain U-3 was a good organism for the production and accumulation of xylooligosaccharideswith long chains from xylan by a microbial culture. Xylanase produced by strain U-3 was purified to homogeneity and characterized.The hydrolyzates generated by the purified xylanase contained xylobiose, xylotrinse, xylotewaose, and xylopentaose, but not xylose. |
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Keywords: | xyloofigosaccharides mutant strain xylananase |
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