Improved regeneration response of creeping bentgrass and japonica rice by maltose and lactose |
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Authors: | Yoshito Asano Yasuyuki Ito Mari Ohara Kiyoyuki Sugiura Azusa Fujiie |
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Affiliation: | (1) Faculty of Horticulture, Chiba University, 277 Kashiwa, Japan;(2) Chiba Prefectural Agricultural Experiment Station, 280-02 Chiba, Japan;(3) Japan Turfgrass Inc., 275 Narashino, Japan |
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Abstract: | The effect of alternative carbohydrate sources to sucrose for plant regeneration from long-term cell cultures of creeping bentgrass (Agrostis palustris Huds.cv.Penncross) and japonica rice (Oryza sativa L.cv.Nipponbare) was studied. Both maltose and lactose supported a higher degree of regeneration compared to sucrose; in 8-and 19-month-old cultures of creeping bentgrass, the frequencies of regenerating calli remained at 76–93% and the numbers of plants regenerated were 8 to 36-fold higher. In 35-month-old cultures of japonica rice, 2–4% of the calli were capable of regeneration on maltose and lactose media. These results indicate that loss of plant regeneration in long-term cultures is caused, at least in part, by specific cultural conditions and not by genetic changes. |
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Keywords: | long-term cultures |
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