Modulation of SCE induction and cell proliferation by 2-mercaptoethanol in phytohemagglutinin-stimulated rat lymphocytes |
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Authors: | Anane Aidoo Suzanne M Morris Olen E Domon Lynda J Mc Garrity Ralph L Kodell Daniel A Casciano |
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Institution: | (1) Divsion of Genetic Toxicology, and Biometry Staff, National Center for Toxicological Research, 72079 Jefferson, Arkansas |
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Abstract: | 2-Mercaptoethanol (2-ME) is used as a medium supplement to enhance the proliferation of lymphocytes culturedin vitro. In this study, we have examined the effects of 2-ME on cell growth and on SCE induction in cultures of unstimulated and phytohemagglutinin (PHA)-stimulated Fischer 344 rat lymphocytes. There were virtually no metaphases detected in cells cultured without PHA. In PHA-stimulated cultures, 2-ME decreased SCE-frequency but it enhanced SCE frequency in the presence of S to 12.5 µM bromodeoxyuridine (BRd U). Both mitotic and replication indices were increased in the PHA/2-ME system. The levels of incorporated exogenous thymidine, in the presence of 2-ME, were relatively low in unstimulated cells, suggesting that 2-ME is not mitogenic for T-cells. However, 2-ME enhanced PHA-induced response of T-cells as evidenced by increased levels of thymidine incorporation into cellular DNA. The growth promoting effects and the decrease in SCE frequency caused by 2-ME upon PHA stimulation indicate that 2-ME may alter the nature of interaction between PHA and cellular activating properties or the replicative processes.Abbreviations BRdU
bromodeoxyuridine
- FBS
fetal bovine serum
- SCE
sister-chromatid exchanges
- HEPES
N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid
- IL-2
interleukin-2
- 2-ME
2-mercaptoethanol
- PBS
phosphate buffered saline
- PHA
phytohemagglutinin
- MI
mitotic index
- RI
replication index
- NADH
nicotinamide adenine dinucleotide (reduced form) |
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Keywords: | 2-Mercaptoethanol PHA-stimulated rat lymphocytes growth promoting effects sister-chromatid exchange frequency cell proliferation |
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