Transgenic tobacco resistant to a bacterial disease by the detoxification of a pathogenic toxin |
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Authors: | Hiroyuki Anzai Katsuyoshi Yoneyama Isamu Yamaguchi |
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Affiliation: | (1) Microbial Toxicology Laboratory, The Institute of Chemical and Physical Research (RIKEN), 2-1 Hirosawa, 351-01 Wako, Japan;(2) Present address: Pharmaceutical Research Center, Meiji Seika Kaisha, Ltd., 222 Morooka-cho, Kohoku-ku, Yokohama, Japan |
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Abstract: | Summary Some plant pathogens produce toxins which cause disease in infected plants. One of the pathogenic toxins, tabtoxin, is produced by Pseudomonas syringae pv. tabaci, which causes wildfire of tobacco. A tabtoxin resistance gene (ttr) coding for an acetyltransferase isolated from Pseudomonas syringae pv. tabaci was fused to the 35S promoter of the cauliflower mosaic virus (CaMV) to construct a chimeric gene for introduction into tobacco cells by Agrobacterium-mediated transformation. The transgenic tobacco plants showed high specific-expression of the ttr gene and no chlorotic symptoms caused by tabtoxin treatment or with infection by Pseudomonas syringae pv. tabaci. These results demonstrate a successful approach to obtain disease-resistant plants by detoxification of the pathogenic toxins which play an important role in pathogenesis. |
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Keywords: | Transgenic plant Resistance Tabtoxin Wildfire disease |
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