Development of a polymerase chain reaction assay for the detection of Listeria monocytogenes in foods |
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Authors: | N.S. Bansal |
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Affiliation: | Molecular Microbiology Laboratory, Division of Analytical Laboratories, NSW Health Department, Lidcombe, NSW, Australia |
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Abstract: | N.S. BANSAL. 1996. Species-specific oligonucleotide primers were selected from the coding region of the listeriolysin O gene of Listeria monocytogenes and were used in conjunction with genus-specific primers and an internal control fragment for polymerase chain reaction amplification. The specificity of the primers was confirmed by testing 40 isolates of L. monocytogenes , other Listeria species and other micro-organisms which are ubiquitous in the environment. The reliability of these primers was further tested in parallel with standard cultural methods. In a preliminary study, over 250 different food samples were examined and PCR results were in complete agreement with those obtained from standard cultural procedures. |
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