| 双歧杆菌粘附大肠癌细胞相关因素探讨 |
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| 引用本文： | 郭骏,车济国.双歧杆菌粘附大肠癌细胞相关因素探讨[J].中国微生态学杂志,1998,10(5):273-275. |
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| 作者姓名： | 郭骏 车济国 |
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| 作者单位： | 大连医科大学组胚教研室，江西抚州地区供电局医院 |
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| 摘 要： | 本文以青春双歧杆菌ＤＭ９２２７粘附大肠癌细胞ｃｌ－１８７为实验模型，探讨粘附的相关因素。结果表明：粘附需要一定的菌浓度（＞１０６ＣＦＵ／ｍｌ），一定的温度（３７℃）及一定的时间（＞１０ｈ），且偏酸更有利于粘附。破坏菌体表面的糖结构使粘附上升，而破坏蛋白结构则使粘附下降。
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| 关 键 词： | 双歧杆菌 粘附 |
FACTORS INVOLVED IN ADHERENCE OF BIFIDOBACTERIA TO HUMAN COLON CARCINOMA CELLS |
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| GuoJun,et. National Laboratory of Medical Neurobiology and.FACTORS INVOLVED IN ADHERENCE OF BIFIDOBACTERIA TO HUMAN COLON CARCINOMA CELLS[J].Chinese Journal of Microecology,1998,10(5):273-275. |
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| Authors: | GuoJun et National Laboratory of Medical Neurobiology and |
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| Institution: | GuoJun,et. National Laboratory of Medical Neurobiology and Department of Neurobiology,Shanghai Medical university,138 Yi Xue Yuan Road.Shang Hai 200032 |
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| Abstract: | The quantitative assay was performed to investigate factors involved in the adherence of Bifidobacteria to cultured CCL 187 cells. The results Showed:Adherencl was concentration and temperature dependent,greater at acid pH values and saturated after co incubation for one hour.Moreover,it was decreased by treatment of rypsin on the bacterial surface proteins but enhanced by treatment of periodate on the carbohydrates,Which indicate that protein and carbohydrate on the bacterial surface were important for the adherence of this strain to CCL 187 cells.Rather,a pH factor in spent culture supernatant contributed significantly to the adherence of this strain. |
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| Keywords: | Bifidobacteria Adhernce |
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