| 紫外线诱变豇豆根瘤病512的研究 |
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| 引用本文： | 秦家顺,吴晓辉.紫外线诱变豇豆根瘤病512的研究[J].中国微生态学杂志,1998,10(1):24-26. |
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| 作者姓名： | 秦家顺 吴晓辉 |
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| 作者单位： | 重庆市涪陵师专，四川师范大学，四川乐山师专 |
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| 摘 要： | 将活化后的豇豆根瘤菌５１２（Ｒ．Ｉｅｇｕｍｉｎｏｓａｒｍ５１２）涂抹于含有１ｇ／Ｌ琥珀酸钠和０．８％蛋白胨的ＹＥＭ固体培养基上，然后以紫外线照射，从中选出合成ＩＡＡ能力较高和抗性较强的突变菌株。测定结果表明，该菌株合成ＩＡＡ成力比原出发菌株高一倍左右。其合成ＩＡＡ能力与照射剂量、照射次数和培养基中蛋白胨的浓度有关。不仅如此，该突变菌株的耐碱和耐盐能力有明显提高。
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| 关 键 词： | 豇豆根瘤菌512 紫外线 诱变 |
STUDY ON INDUCED MUTATION OF R. LEGUMINOSARM512 WITH ULTRAVIOLET IRRADIAION |
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| Abstract: | R.Ieguminosarm512 activated were smeared on YEM solid medium with 1g/L sodium succinate 0.8% peptone. After irradiation with ultraviolet, a mutational strain of high IAA production and strong enviroment-resistance was obtained. The resuets showed that the ability of IAA production of the mutational strain is one time higher than the original one, and is related to the dosage and number of tine irradiation with ultraviolet,the conentration of peptone in medium.It is nat so,but their alkali-resistance and salt-resistance was obviously raised. |
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| Keywords: | R Ieguminosarm512 Ultraviolet Induction |
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