Demonstration of sulfhydryl and disulfide groups by a fluorescent maleimide procedure |
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Authors: | Sherill K Curtis Ronald R Cowden |
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Institution: | (1) Department of Anatomy and Program in Cellular Biophysics, College of Medicine, East Tennessee State University, 37614 Johnson City, TN, USA |
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Abstract: | Summary Several fluorescent maleimide compounds were evaluated as possible substitutes for N-(4-aminophenyl)maleimide in the histochemical procedures developed by Sippel (1973, 1978a, b, 1980) for the demonstration of sulfhydryl and disulfide groups. The brightest and most selective fluorescence was obtained by using N-(7-dimethylamino-4-methylcoumarinyl)maleimide (DACM), although both eosin-5-maleimide and fluorescein-5-maleimide could also be used if adequate control preparations were made. |
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