用ISSR标记检测黄麻野生种与栽培种遗传多样性

利用ISSR标记对黄麻属((Corchorus)10个种27份材料的遗传多样性进行分析，25个ISSR引物共扩增出283条带，平均每个引物扩增出10．48条带，多态性条带比例(PPB)为92．85％；种间遗传相似系数在0．33～0．97之间。表现出丰富的遗传多样性．系统聚类结果显示，第I、Ⅲ类群均为黄麻属8个种11份原始野生种，种间存在丰富的遗传多样性；第Ⅱ类群为黄麻两个栽培种及其近缘野生种，共16份材料。种遗传相似性较高；基因组聚类结果与经典分类相符．利用分子标记技术研究初步可以认为。荨麻叶种为最原始的黄麻野生种之一；三室种21C为三室种的一个变种；甜麻为一个尚待定性的野生种．同组材料的RAPD和ISSR分析结果，具有较高的相似性和可信度．

Application of ISSR technology in genetic diversity detection of Jute

The genetic diversity among 27 accessions of Corchorus, including 10 Jute species, was investigated with ISSR technique. 283 DNA bands were amplified with 25 ISSR primers, among which, 263 (92.85%) were polymorphic, with 10.48 bands per primer in average. A further systemic cluster analysis indicated that the accessions could be clustered into three groups, and the group II (including two cultispecies and their close wild species) was obviously genetically different from the groups I and III (including eight wild species). Moreover, 16 accessions in group II presented a higher intraspecific genetic resemblance, while 11 accessions among groups I & III showed an abundant interspecific genetic diversity. After synthesized the relevant findings of morphology and DNA classification, it's found that C. urticifolius could be one of the original wild species, C. tilaculariszic was a variation of C. tilaculari, and Tian Jute could be an untitled wild species.