Lineage Analysis Using Retroviral Vectors |
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| Authors: | Constance L. Cepko Elizabeth Ryder Christopher Austin Jeffrey Golden Shawn Fields-Berry John Lin |
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| Affiliation: | aDepartment of Genetics, Harvard Medical School and Howard Hughes Medical Institute, Boston, Massachusetts, 02115;bDepartment of Biology and Biotechnology, Worcester Polytechnic Institute, Worcester, Massachusetts, 01609;cMerck Research Laboratories, West Point, Pennsylvania, 19486;dDepartment of Pathology, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, 19104 |
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| Abstract: | Knowledge of the genealogical relationships of cells during development can allow one to gain insight into when and where developmental decisions are being made. Genealogical relationships can be revealed by a variety of methods, all of which involve marking a progenitor cell and/or a group of cells and then following the progeny. The use of replication-incompetent retroviral vectors for the analysis of lineal relationships in developing vertebrate tissues is described. An overview of the relevant aspects of the retroviral life cycle is given, and the strategies and current methods in use in our laboratory are described. |
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