Depressor effect of diabetes in spontaneously hypertensive rat: role of vascular reactivity and prolyl hydroxylase and lysyl oxidase activities

Streptozotocin (STZ)-induced diabetes (8 weeks) produced a marked depressor effect in the spontaneously hypertensive rat (SHR), confirming earlier studies, but had no effect on arterial pressure of normotensive controls (WKY). We investigated the phenomenon further by examining the effects of diabetes on the activities of aortic prolyl hydroxylase (PH) and lysyl oxidase (LO), marker enzymes for collagen biosynthesis, and on the reactivity of isolated mesenteric arteries to vasoactive agents. PH and LO activities of nondiabetic SHR were greater than those of the WKY controls. Diabetes markedly reduced PH and LO activities of SHR aortae, but had no significant effect on PH and LO activities of the WKY strain. The effects of diabetes on vascular collagen biosynthetic enzymes of SHR were not associated with reductions in mesenteric arterial responsiveness or sensitivity to norepinephrine, methoxamine, serotonin or KC1. These results suggest that the depressor effect of diabetes in SHR is associated with a reduction in vascular collagen biosynthesis but not a reduction in vascular reactivity.