滑子菇多糖的体外生物活性初步研究

为了研究滑子菇水提粗多糖(PNP)的体外生物活性,对滑子菇多糖的总还原力、清除1,1-二苯基-2-苦苯肼自由基(DPPH·)和由Fe2+诱发的脂质过氧化反应的抑制作用进行研究,采用MTT比色法和胎盘蓝细胞计数检测对滑子菇水提粗多糖的体外抑制K562细胞生长作用进行了研究,采用流失细胞术对滑子菇多糖作用人白血病K562细胞后的细胞周期进行了研究。结果表明:滑子菇水提粗多糖PNP具有一定的还原能力;在高质量浓度(800μg/mL)时具有接近于Vc清除DPPH·的能力,达41.28%;PNP对Fe2+诱发的脂质过氧化反应具有一定的抑制作用,并且随着浓度的增加抑制作用逐渐增强,但总的增长趋势不大;MTT实验表明PNP对K562细胞的体外增长有抑制作用,在质量浓度为800μg/mL和作用时间为48 h时,可达到最高的抑制率35.03%。流式细胞术对细胞周期的检测表明滑子菇多糖能够阻滞人白血病K562细胞于G1期。

In vitro biological activity of polysaccharide extracted from Pholiota nameko

Fungus polysaccharide had an important value of biological activity,Pholiota nameko was plant fungus of edible medicinal value. Water-extracted crude polysaccharides of Pholiota nameko, were investigated. Their antioxidant activity was studied by DPPH method, the lipid peroxidation by Fe2＋method,the antitumor activity by MTT assay,and placenta blue cell count detection.The flow cytometer was used to study effects of PNP on K562 cell cycle.Experimental results showed that PNP had inhibitory ability and DPPH free radical scavenging capability similar to that of Vc on high concentration.PNP had actions on lipid peroxidation with the increasing of the concentration,the inhibitory effect increased.MTT assay showed that PNP inhibited the growth of K562 cells in vitro,exhibited dose-dependent and time-based effect,the highest inhibition rate is 35.03% when treated with PNP in 800 μg/mL for 48 h.In addition,the result of cell cycle analysis by flow cytometer showed that PNP could block the K562 cell in G1 phase.