| 微生物油脂中花生四烯酸含量的准确快速测定 |
| |
| 引用本文： | 颜佳铖,纪晓俊,聂志奎,邓中涛,任路静,黄和. 微生物油脂中花生四烯酸含量的准确快速测定[J]. 生物加工过程, 2014, 0(2): 66-69 |
| |
| 作者姓名： | 颜佳铖 纪晓俊 聂志奎 邓中涛 任路静 黄和 |
| |
| 作者单位： | 南京工业大学 生物与制药工程学院材料化学工程国家重点实验室,南京210009 |
| |
| 基金项目： | 国家高技术研究发展计划（863计划）（2014AA021703）；国家自然科学基金（21376002）；江苏省自然科学基金（BK20131405）；江苏高校优势学科建设工程 |
| |
| 摘 要： | 建立了快速、准确测定微生物油脂中花生四烯酸(ARA)含量的气相色谱检测方法。选用DB-23毛细管色谱柱,设置合适的载气压力,采用FID检测器,对ARA含量进行了定量分析。测定结果表明:油脂中各脂肪酸组分可有效分离,分析时间仅需20 min,ARA的回收率为90.146%~100.634%,相对标准偏差为4.175%。
|
| 关 键 词： | ARA 气相色谱分析 微生物油脂 |
Fast determination of arachidonic acid in microbial oils |
| |
| YAN Jiacheng,JI Xiaojun,NIE Zhikui,DENG Zhongtao,REN Lujing,HUANG He. Fast determination of arachidonic acid in microbial oils[J]. Chinese Journal of Bioprocess Engineering, 2014, 0(2): 66-69 |
| |
| Authors: | YAN Jiacheng JI Xiaojun NIE Zhikui DENG Zhongtao REN Lujing HUANG He |
| |
| Affiliation: | YAN Jiacheng, JI Xiaojun, NIE Zhikui, DENG Zhongtao, REN Lujing, HUANG He, YAN Jiacheng, JI Xiaojun, NIE Zhikui, DENG Zhongtao, REN Lujing, HUANG He |
| |
| Abstract: | Gas chromatography was performed for the determination of arachidonic acid （ ARA） produced by micro-organisms.Capillary column DB-23 was used with FID and suitable carrier gas for the detection.The result indicated that the method could separate fatty acids well in 20 min on good precision and recovery.The recovery rate of ARA was 90.146% to 100.634%,and the relative standard deviation was 4.175%.Thus,the method was effective for the detection of ARA produced by micro-organisms. |
| |
| Keywords: | ARA arachidonic acid gas chromatography microbial oils ARA arachidonic acid gas chromatography microbial oils |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
