高抗逆高丁比拜氏梭菌的选育及其性能考察简

以抗逆突变株Clostridium beijerinckii IB4为出发菌株，通过常压室温等离子体诱变（ ARTP ），刃天青平板初筛，摇瓶发酵复筛，筛选出1株高抗逆高丁比的突变菌株C.beijerinckii IT111。发酵结果表明：该突变菌株利用多种C源时均展现其高丁醇比的特性，以玉米芯酸解糖液为C源时，溶剂产量达到10.5 g/L，丁醇8.0 g/L，丁醇比高达76％。抑制物抗逆性测试结果显示：糠醛和酸类对C.beijerinckii发酵影响较小，酚类物质对C.beijerinckii抑制作用较强，其中以香草醛为最。综上所述，C.beijerinckii IT111是1株极具潜力的利用木质纤维原料制备丁醇的菌株。

Screening of Clostridium beijerinckii mutant with high inhibitor tolerance and high proportions of butanol for butanol production

Clostridium beijerinckii IT111,a tolerance mutant strain with high proportions of butanol,was obtained by atmospheric and room temperature plasma （ ARTP ） and high-throughput screening.Fermentation results showed that the mutant had a high butanol proportions using a variety of carbon sources.When non-detoxified hemicellulosic hydrolysate of corn fiber treated with dilute sulfuric acid （ SAHHC） was used as a substrate,total solvent（ ABE） and butanol production of 10.5 g/L and 8.0 g/L were obtained,respectively.The butanol proportion was up to 76%.The results of toxicity evaluation of model inhibitors showed that acetates,furfural and 5-HMF were not inhibitory to ABE production,while phenolic compounds were potent inhibitors of growth and ABE production, especially vanillin.C.beijerinckii IT111 was a promising strain for ABE production from lignocellulosic materials.