| 利用生物柴油副产物粗甘油生产二羟基丙酮 |
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| 引用本文： | 郑小娟,;孙杨,;陈万河,;靳魁奇,;潘龙,;刘宇鹏.利用生物柴油副产物粗甘油生产二羟基丙酮[J].生物加工过程,2014(4):7-10. |
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| 作者姓名： | 郑小娟 ;孙杨 ;陈万河 ;靳魁奇 ;潘龙 ;刘宇鹏 |
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| 作者单位： | [1]河南大学 生命科学学院 生物工程研究所,开封475004; [2] 保定九孚生化有限公司,保定071052 |
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| 基金项目： | 河南省科技厅攻关项目（132102210387）；2011年度河南大学青年骨干教师培养计划 |
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| 摘 要： | 以来自餐饮废油的生物柴油副产物粗甘油作为廉价底物,对弗托氏葡糖杆菌(Gluconobacter frateurii)CGMCC5397发酵转化生产二羟基丙酮(DHA)进行初步研究。研究发现粗甘油中的金属离子,尤其是Zn2+对微生物转化生产二羟基丙酮有明显抑制作用。粗甘油经过预处理后,利用优化后的发酵培养基,在7 L发酵罐中进行补料分批发酵,48 h后DHA浓度达到89.5 g/L,生产强度为1.86 g/(L·h),甘油转化率为90.1%。本研究初步证明了弗托氏葡糖杆菌能高效和经济地利用生物柴油副产物粗甘油生产DHA。
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| 关 键 词： | 生物柴油 二羟基丙酮 甘油 Zn+ 弗托氏葡糖杆菌 |
Dihydroxyacetone producted by biodiesel-derived crude glycerol |
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| Institution: | ZHENG Xiaojuan, SUN Yang, CHEN Wanhe,JIN Kuiqi, PAN Long, LIU Yupeng ( 1. Institute of Bioengineering, School of Life Sciences, Henan University, Kaifeng 475004, China; 2. Baoding Jiufu Biochem Co. Ltd., Baoding 071052, China) |
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| Abstract: | The production of dihydroxyacetone by Gluconobacter frateurii （ CGMCC5397 ） growing on biodiesel-derived crude glycerol as substrate was investigated. Analysis of different ions in crude glycerol revealed that Zn2＋ showed a significant inhibitory effect on the production of dihydroxyacetone by microbial. After pretreatment of crude glycerol, DHA fermentations were carried in bioreactor. When fed-batch fermentation was carried out in a 7-L stirred bioreactor, the DHA concentration, productivity, and yield were 89.5 g/L, 1.86 g/（ L·h ） , and 90.1% at 48 h, respectively. The study proved that the biodiesel-derived crude glycerol could be utilized by G. frateurii for efficient and economical production of DHA. |
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| Keywords: | biodiesel dihydroxyacetone glycerol zinc Gluconobacter frateurii |
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