| 壳聚糖絮凝法精制合欢皮多糖水提液的工艺 |
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| 引用本文： | 韩乐,孙晓海,韩伟.壳聚糖絮凝法精制合欢皮多糖水提液的工艺[J].生物加工过程,2014(2):38-43. |
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| 作者姓名： | 韩乐 孙晓海 韩伟 |
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| 作者单位： | 华东理工大学 中药现代化工程中心,上海200237 |
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| 基金项目： | 国家大学生创新计划（111025124） |
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| 摘 要： | 采用壳聚糖对合欢皮总多糖的絮凝纯化工艺进行研究。通过单因素和正交试验,得到优化的工艺条件:壳聚糖用量为生药量0.8 mL/g,絮凝温度35℃,絮凝时间4 h,药液浓缩比(质量体积比)为1∶10(g/mL),在此条件下,多糖保留率、脱蛋白率和多糖纯度分别为91.23%、22.26%和47.27%,优于传统的水提醇沉法。
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| 关 键 词： | 合欢皮 壳聚糖 絮凝 多糖 |
Flocculating purification process of total polysaccharides from bark of Albizzia julibrissin by using chitosan |
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| HAN Le,SUN Xiaohai,HAN Wei.Flocculating purification process of total polysaccharides from bark of Albizzia julibrissin by using chitosan[J].Chinese Journal of Bioprocess Engineering,2014(2):38-43. |
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| Authors: | HAN Le SUN Xiaohai HAN Wei |
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| Institution: | HAN Le, SUN Xiaohai, HAN Wei, HAN Le, SUN Xiaohai, HAN Wei |
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| Abstract: | Through single factor and orthogonal experimental designs, the optimun conditions were obtained as follows：the dosage of chitosan was 0.8 mL/g, temperature 35 ℃, flocculation time 4 h, determined as 1∶10 of drug density.Under the optimum conditions,polysaccharide retention rate,protein removal rate,and the purity of polysaccharide were obtained as follows：91.23%,22.26% and 47.27%, respectively.The flocculating purification of total polysaccharides was superior to the traditional method of extracting with water and depositing with alcohol. |
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| Keywords: | Albizzia julibrissin chitosan flocculating polysaccharide Albizzia julibrissin chitosan flocculating polysaccharide |
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