PCR and real-time PCR primers developed for detection and identification of <Emphasis Type="Italic">Bifidobacterium thermophilum</Emphasis>in faeces |
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Authors: | Sophie Mathys Christophe Lacroix Raffaella Mini Leo Meile |
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Institution: | (1) Laboratory of Food Biotechnology, Institute of Food Science and Nutrition, ETH Zurich, Switzerland |
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Abstract: | Background Culture-independent methods based on the 16S ribosomal RNA molecule are nowadays widely used for assessment of the composition
of the intestinal microbiota, in relation to host health or probiotic efficacy. Because Bifidobacterium thermophilum was only recently isolated from human faeces until now, no specific real-time PCR (qPCR) assay has been developed for detection
of this species as component of the bifidobacterial community of the human intestinal flora. |
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