DNA-Binding Activities in Streptococcus gordonii: Identification of a Receptor-Nickase and a Histonelike Protein |
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| Authors: | R Dwayne Lunsford Nga Nguyen J London |
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| Institution: | (1) Laboratory of Microbial Ecology, National Institute for Dental Research, National Institutes of Health, Bldg 30, Rm 312, 30 Convent Drive, Bethesda, MD 20892, USA , US;(2) Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA , US |
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| Abstract: | Extraction of Streptococcus gordonii cells with the mild chaotropic agent, LiCl, drastically decreased DNA transforming ability, had little effect on viability,
and released both DNA nicking and binding activities. Both activities were Mg2+ and Ca2+ independent and were not competence specific. Southwestern blot analysis of the extract identified putative surface proteins
of 56 kDa and 68 kDa in strain Challis and Wicky, respectively. Extracts also contained a 10-kDa DNA-binding protein, designated
HSgo, that belongs to the eubacterial histonelike class of proteins. |
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