High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore |
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Authors: | Chengzu Xiao Zicai Huang Wengqing Li Xianwen Hu Wenlu Qu Lihua Gao Gaoyan Liu |
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Institution: | (1) Department of Cell Engineering, Institute of Biotechnology, 20 Dongdajie, Fengtai, Beijing, 100071, P.R. China |
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Abstract: | Using porous microcarrier Cytopore and a low-serum medium supplement BIGBEF-3, we have successfully cultivated recombinant
CHO cell line CL-11G producing prourokinase and hybridomas producing anti-prourokinase monoclonal antibody in Celligen 1.5
or 5 L bioreactor. The cell density obtained ranged from 1 to 2 × 107 cells mL-1. The yields of prourokinase and monoclonal
antibody increased with increasing cell density. As the cells could spontaneously release from and reattach to porous microcarriers,
it was very easy to scale-up the cultivation. Thus the bead to bead cell transfer method has been used to scale up the cultivation
of CL-11G cells to a 20 L reactor-scale for the pilot production of prourokinase, and also to scale-up the culture of hybridomas
for the production of monoclonal antibody for the purification of prourokinase.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | monoclonal antibody porous microcarrier prourokinase recombinant CHO cells |
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