Characterization of siderophores from Ustilago maydis |
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| Authors: | Allen D. Budde Sally A. Leong |
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| Affiliation: | (1) USDA/ARS, Plant Disease Resistance Research Unit, Department of Plant Pathology, University of Wisconsin, 53706 Madison, WI, USA |
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| Abstract: | Two siderophores, ferrichrome and ferrichrome A, were found in cultures of Ustilago maydis (DC) Corda. Both siderophores were found intracellularly and extracellularly. Their authenticity was confirmed by thin layer chromatography, HPLC, UV-visible spectrometry, paper electrophoresis, amino acid analysis, NMR and fast atom bombardment mass spectroscopy. Regulation of siderophore production by iron was examined. Repression of biosynthesis of extracellular siderophores occurred at 10–5 M iron. Ferrichrome was found intracellularly at all iron concentrations employed; in general, ferrichrome A was not found to be cell-associated. |
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| Keywords: | ferrichrome ferrichrome A hydroxamate siderophore and Ustilago maydis |
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