Biochemical and histological changes associated with <Emphasis Type="Italic">in vitro</Emphasis> responses in sunflower cotyledonary explants |
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Authors: | T A Vega G M Nestares G Pratta R Zorzoli S Gattuso L Picardi |
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Institution: | (1) Cátedra de Genética, Fac. Cs. Agrarias, Universidad Nacional de Rosario, Campo Experimental Villarino CC 14, 2125 Zavalla, Argentina;(2) Cátedra de Botánica, Fac. Cs. Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Santa Fe, Argentina |
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Abstract: | In vitro shoot regeneration from sunflower cotyledonary explants can be obtained in the presence of kinetin and indole-3-acetic acid.
In contrast, callus proliferation is obtained in the presence of 2,4-dichlorophenoxyacetic acid on culture medium. The purpose
of this study was to investigate changes in protein profiles during callus and shoot development from cotyledonary explants
and to correlate them with ontogenic stages during in vitro culture. Cotyledons cultured in the presence of 2,4-dichlorophenoxyacetic acid produced friable callus as a result of early
division of parenchymatic cells associated with the vascular bundles of the explant. The callogenic ability was independent
of the cotyledonary region used as starting explant. Direct shoot organogenesis was observed from the same type of cells growing
in culture media supplemented with kinetin and indole-3-acetic acid. In this case, the regeneration potential varied among
regions from which the explants were obtained. Protein profiles revealed differences associated with shoots or callus developmental
programs. A 27-kDa polypeptide was uniquely detected in the explants undergoing shoot organogenesis. The amount of this polypeptide
during the first 4 d of culture increased and was followed by the appearance of meristematic centers in histologically analyzed
samples. This polypeptide could be used as a specific marker for in vitro shoot development in this species. |
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Keywords: | Direct organogenesis Protein profiles Callus growth Helianthus annuus L Regeneration |
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