In vitro propagation of Bambusa nutans Wall. ex Munro through axillary shoot proliferation |
| |
Authors: | Divya Negi Sanjay Saxena |
| |
Institution: | (1) TERI University, 10 Institutional Area, Vasant Kunj, New Delhi, 110070, India;(2) The Energy and Resources Institute (TERI), IHC Complex, Lodhi Road, New Delhi, 110003, India |
| |
Abstract: | This communication describes for the first time an efficient and reproducible protocol for large-scale multiplication of Bambusa nutans. Nodal segments collected from field-grown clumps and cultured on Murashige and Skoog (MS) medium supplemented with 4.4 μM
benzylaminopurine (BA) and 2.32 μM kinetin (Kin) gelled with 0.2% gelrite yielded 80% aseptic cultures with 100% bud-break.
The in vitro-formed shoots obtained after bud-break were successfully multiplied in MS liquid medium supplemented with 13.2 μM
BA, 2.32 μM Kin, and 0.98 μM indole-3-butyric acid (IBA). Sub-culturing of shoots every 3 weeks on fresh multiplication medium
yielded a consistent proliferation rate of 3.5-fold. Shoot clusters containing three to five shoots were successfully rooted
with 100% success on half-strength MS liquid medium supplemented with 9.8 μM IBA, 2.85 μM indole-3-acetic acid (IAA), 2.68 μM
naphthaleneacetic acid (NAA), and 3% sucrose. Plantlets grown in vitro were acclimatized and subsequently transferred to the
field. Inter-simple sequence repeat analysis has confirmed the genetic uniformity of the tissue-cultured plants up to 27 passages. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|