Synthesis, characterization, cytotoxic activity and DNA binding properties of the novel dinuclear cobalt(III) complex with the condensation product of 2-acetylpyridine and malonic acid dihydrazide |
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| Authors: | Eshkourfu Rabia Čobeljić Božidar Vujčić Miroslava Turel Iztok Pevec Andrej Sepčić Kristina Zec Manja Radulović Siniša Srdić-Radić Tatjana Mitić Dragana Andjelković Katarina Sladić Dušan |
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| Affiliation: | a Faculty of Chemistry, University of Belgrade, Studentski trg 12-16, 11000 Belgrade, Serbiab Institute of Chemistry, Technology and Metallurgy, University of Belgrade, Njegoševa 12, P.O. Box 815, 11000 Belgrade, Serbiac Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ašker?eva 5, 1000 Ljubljana, Sloveniad Biotechnical Faculty, University of Ljubljana, Department of Biology, Ve?na pot 111, 1000 Ljubljana, Sloveniae Institute for Oncology and Radiology of Serbia, Department of Experimental Oncology, Laboratory for Experimental Pharmacology, Pasterova 14, 11000 Belgrade, Serbia |
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| Abstract: | A novel dinuclear cobalt(III) complex with the condensation product of 2-acetylpyridine and malonic acid dihydrazide, N′,N′2-bis[(1E)-1-(2-pyridyl)ethylidene]propanedihydrazide was synthesized and characterized by elemental analysis, spectroscopy (NMR and infrared), and X-ray crystal analysis. The complex showed a moderate activity towards Artemia salina. The highest cytotoxic potential of the complex was observed on the epithelial breast cancer (MDA-361) cell line. The investigated complex induced apoptosis, the early apoptotic cells comprising 28.18%, compared to 5.64% of control cells in the same phase. The interaction of the complex with calf thymus DNA (CT-DNA) was monitored by blue shift and hyperchromism in the UV-vis spectra. The observed intrinsic binding constant (Kb = 4.2 × 105 M− 1) together with structural analysis of the complex indicate the groove binding. |
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| Keywords: | Dinuclear cobalt(III) complex Crystal structure Apoptosis DNA binding |
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