Peptide enzymatic microsynthesis, using carboxypeptidase Y as the catalyst: application to stepwise synthesis of Leuenkephalin |
| |
Authors: | F Hellio P Gueguen J L Morgat |
| |
Institution: | Centre d'Etudes Nucléaires de Saclay, Département de Biologie, Service de Biochimie, C.E.A., Gif-sur-Yvette, France. |
| |
Abstract: | In order to develop the use of carboxypeptidase Y (CPD-Y, EC 3.4.12.1) as a catalyst for radioactive hormonal synthesis, the stepwise synthesis of a pentapeptide, Leu-enkephalin, was studied on a microscale. Each peptide bond was formed by enzymatic catalysis, using microquantities of the precursors (amino acid or peptide esters as acyl-components and amino acid ester or amide as nucleophiles). The high condensation yields obtained suggests that CPD-Y can be a useful tool for preparation of radioactive hormonal peptides. |
| |
Keywords: | |
|
|