Formylmethanofuran: tetrahydromethanopterin formyltransferase and N 5,N 10-methylenetetrahydromethanopterin dehydrogenase from the sulfate-reducing Archaeoglobus fulgidus: similarities with the enzymes from methanogenic Archaea |
| |
| Authors: | B. Schwörer J. Breitung A. R. Klein K. O. Stetter R. K. Thauer |
| |
| Affiliation: | (1) Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität Marburg, Karl-von-Frisch-Strasse, W-3550 Marburg/Lahn, Germany;(2) Max-Planck-Institut für Terrestrische Mikrobiologie Marburg, Karl-von-Frisch-Strasse, W-3550 Marburg/Lahn, Germany;(3) Lehrstuhl für Mikrobiologie, Universität Regensburg, Universitätsstrasse 31, W-8400 Regensburg, Germany |
| |
| Abstract: | The sulfate-reducing Archaeoglobus fulgidus contains a number of enzymes previously thought to be unique for methanogenic Archaea. The purification and properties of two of these enzymes, of formylmethanofuran: tetrahydromethanopterin formyltransferase and of N5,N10-methylenetetrahydromethanopterin dehydrogenase (coenzyme F420 dependent) are described here. A comparison of the N-terminal amino acid sequences and of other molecular properties with those of the respective enzymes from three methanogenic Archaea revealed a high degree of similarity.Abbreviations H4MPT tetrahydromethanopterin - F420 coenzyme - F420 formyltransferase, formylmethanofuran: tetrahydromethanopterin formyltransferase - methylene-H4MPT dehydrogenase N5,N10-methylenetetrahydromethanopterin dehydrogenase - methylene-H4MPT recductase N5,N10-methylenetetrahydromethanopterin reductase - cyclohydrolase N5,N10-methenyltetrahydromethanopterin cyclohydrolase - APS adenosine 5 -phosphosulfate - MOPS 3-(N-morpholino) propane sulfonic acid - TRICINE N-tris(hydroxymethyl)methylglycine - MES morpholinoethanesulfonic acid - 1 U 1  mol/min |
| |
| Keywords: | Archaea Methanogens Sulfate reducers Tetrahydromethanopterin Methanofuran Coenzyme F420 C1-Enzymes |
| 本文献已被 SpringerLink 等数据库收录！ |
|
