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Methods for imaging Shewanella oneidensis MR-1 nanofilaments
Authors:R Ray  LA Fitzgerald  BR Ringeisen
Institution:a Oceanography Division, US Naval Research Laboratory, Bldg 1009, John C. Stennis Space Center, MS 39529, USA
b Chemistry Division, US Naval Research Laboratory, 4555 Overlook Avenue, SW, Washington, DC. 20375, USA
Abstract:Nanofilament production by Shewanella oneidensis MR-1 was evaluated as a function of lifestyle (planktonic vs. sessile) under aerobic and anaerobic conditions using different sample preparation techniques prior to imaging with scanning electron microscopy. Nanofilaments could be imaged on MR-1 cells grown in biofilms or planktonically under both aerobic and anaerobic batch culture conditions after fixation, critical point drying and coating with a conductive metal. Critical point drying was a requirement for imaging nanofilaments attached to planktonically grown MR-1 cells, but not for cells grown in a biofilm. Techniques described in this paper cannot be used to differentiate nanowires from pili or flagella.
Keywords:Nanofilaments  Nanowires  Scanning electron microscopy  Shewanella oneidensis MR-1
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