In vitro anaerobic biofilms of human colonic microbiota |
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| Authors: | Sproule-Willoughby K M Stanton M Mark Rioux K P McKay D M Buret A G Ceri H |
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| Affiliation: | a Biofilm Research Group, Department of Biological Sciences, University of Calgary, 2500 University Drive NW, Calgary, AB, Canada T2N 1N4b Department of Medicine, Division of Gastroenterology and Department of Microbiology and Infectious Disease, University of Calgary, Health Sciences Centre, 3330 Hospital Drive NW, Calgary, AB, Canada T2N 4N1c Gastrointestinal Research Group, Department of Physiology and Pharmacology, the Calvin, Phoebe and Joan Snyder Institute for Infection, Immunity and Inflammation, University of Calgary, AB, Canada T2N 4N1d Inflammation Research Network, University of Calgary, Faculty of Medicine, Health Sciences Centre, 3330 Hospital Drive NW, Calgary, AB, Canada T2N 4N1 |
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| Abstract: | The human gastrointestinal tract hosts a complex community of microorganisms that grow as biofilms on the intestinal mucosa. These bacterial communities are not well characterized, although they are known to play an important role in human health. This study aimed to develop a model for culturing biofilms (surface-adherent communities) of intestinal microbiota. The model utilizes adherent mucosal bacteria recovered from colonic biopsies to create multi-species biofilms. Culture on selective media and confocal microscopy indicated the biofilms were composed of a diverse community of bacteria. Molecular analyses confirmed that several phyla were represented in the model, and demonstrated stability of the community over 96 h when cultured in the device. This model is novel in its use of a multi-species community of mucosal bacteria grown in a biofilm mode of growth. |
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| Keywords: | BBE, Bacteroides bile esculin agar bp, base pair(s) CBA, Columbia blood agar cfu, colony forming units Ct, threshold cycle dATP, deoxyadenosine triphosphate dCTP, deoxycytodine triphosphate ddH2O, double-distilled water dGTP, deoxyguanosine triphosphate DNA, deoxyribonucleic acid dNTP, deoxynucleotide triphosphate dTTP, deoxythymidine triphosphate EDTA, (ethylenedinitrilo)-tetraacetic acid FAA, Fastidious Anaerobe Agar mEA, mEnterococcus agar MRS, de Man, Rogosa and Sharpe OPLS-DAPBS, orthogonal partial least squares discriminant analysis phosphate-buffered saline PCR, polymerase chain reaction PEA, phenylethanol agar qPCR, quantitative polymerase chain reaction sTSY, supplemented tryptic soy with yeast broth TBE, Tris-borate-EDTA T-RF, terminal restriction fragment T-RFLP, terminal restriction fragment length polymorphism |
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