PCR-linked reverse DNA hybridization using oligonucleotide-specific probes of rpoB for identification of Mycobacterium avium and Mycobacterium intracellulare |
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Authors: | Bai Sun-Joon Eum Jin-Seong Park Young-Doo Chung Sang-Ho Kook Yoon-Hoh Hong Seong-Karp |
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Affiliation: | a Department of Anesthesiology and Pain Medicine, Yonsei University College of Medicine, Seoul 120-752, South Koreab Anesthesia and Pain Research Institute, Yonsei University College of Medicine, Seoul 120-752, South Koreac Division of Bio and Health Sciences, Mokwon University, Daejeon 302-729, South Koread Department of Microbiology, Seoul National University College of Medicine, Seoul 110-799, South Koreae Department of Microbiology, College of Medicine, The Catholic University of Korea, Seoul 137-701, South Korea |
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Abstract: | A PCR-linked reverse DNA hybridization method using two different specific rpoB DNA probes (Avp and Intp) of Mycobacterium avium and Mycobacterium intracellulare, respectively, were evaluated for the differentiation and identification of M. avium and M. intracellulare culture isolates. Among the 504 culture isolates tested by this method, 48 strains showed positive results for M. avium and 60 strains showed positive results for M. intracellulare. The other 396 culture isolates showed negative results for both M. avium and M. intracellulare. These results were consistent with those obtained from partial rpoB (306 bp) sequence analysis and biochemical tests. The negative strains obtained by this DNA hybridization method were identified as M. tuberculosis (366 strains), M. peregrinum (11 strains), M. abscessus (9 strains), M. fortuitum (8 strains), and M. flavescens (2 strains) by rpoB DNA sequence analysis. Due to the high sensitive and specific result obtained by this assay, we suggest that this PCR-linked reverse DNA hybridization method using two different specific rpoB DNA probes of M. avium and M. intracellulare would be used for the rapid and precise method for differentiation and identification of M. avium and M. intracellulare. |
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Keywords: | Mycobacterium avium Mycobacterium intracellulare rpoB PCR-linked reverse DNA hybridization |
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