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Rapid enumeration of respiratory active mycobacteria with fluorescent double staining
Authors:Tomoaki Ichijo  Yoko Izumi  Masao Nasu
Affiliation:
  • a Graduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamadaoka, Suita, Osaka 565-0871, Japan
  • b Research Institute for Humanity and Nature, 457-4 Kamigamo-Motoyama, Kita-Ku, Kyoto 603-0047, Japan
  • Abstract:A new method for rapid enumeration of physiologically active mycobacteria was developed by acid-fast bacilli staining (Auramine O) following formazan reduction. Results can be obtained within 90 min by the optimized procedure, while more than one week is required for the widely used culture-dependent approach.
    Keywords:Acid fast bacilli staining   Fluorescent vital staining   Nontuberculous mycobacteria   Respiratory activity   Visualization
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