A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus membrane potential |
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Authors: | Daniel R. Gentry Imogen Wilding Dongzhao Chen Cindy Richards Magdalena Zalacain Michael N. Gwynn |
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Affiliation: | a Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA, United Statesb Department of Molecular Discovery Research, GlaxoSmithKline Pharmaceuticals , Research Triangle Park , NC, United Statesc Quantitative Sciences, GlaxoSmithKline Pharmaceuticals, Research Triangle Park, NC, United States |
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Abstract: | We developed a homogenous microtiter based assay using the cationic dye 3, 3′-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds. |
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Keywords: | Membrane potential Staphylococcus aureus Microtiter Assay |
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