A rapid microtiter plate assay for measuring the effect of compounds on <em>Staphylococcus aureus</em> membrane potential期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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*A rapid microtiter plate assay for measuring the effect of compounds on Staphylococcus aureus* membrane potential**

Authors:	Daniel R Gentry Imogen Wilding Dongzhao Chen Cindy Richards Magdalena Zalacain Michael N Gwynn

Institution:	^a Antibacterial Discovery Performance Unit, Infectious Diseases Center of Excellence in Drug Discovery, GlaxoSmithKline Pharmaceuticals, Collegeville, PA, United States ^b Department of Molecular Discovery Research, GlaxoSmithKline Pharmaceuticals , Research Triangle Park , NC, United States ^c Quantitative Sciences, GlaxoSmithKline Pharmaceuticals, Research Triangle Park, NC, United States

Abstract:	We developed a homogenous microtiter based assay using the cationic dye 3, 3′-Diethyloxacarbocyanine iodide, DiOC₂(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.

Keywords:	Membrane potential Staphylococcus aureus Microtiter Assay
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