Study of the initial phase of biofilm formation using a biofomic approach |
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Authors: | C. Nagant |
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Affiliation: | Department of Pharmaceutical Microbiology Institute of Pharmacy, Free University of Brussels, Belgium |
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Abstract: | AimsThe purpose of this work was to study the initial steps of formation of a biofilm using the BioFilm Ring Test® and the Crystal violet staining technique.Methods and resultsEight strains of Pseudomonas aeruginosa were studied. The two methods revealed that four strains formed a rapid biofilm. The biofilm formed by these strains was detected after only 45 min with the BioFilm Ring Test® and after 6 h with the Crystal violet method. The enumeration of bacteria of the PA01 strain confirmed that, after 30 min, a significant amount of bacteria had attached on the bottom of the culture wells. After 48 h the Crystal violet method detected a biofilm with all strains. The four strains which rapidly formed a biofilm did not differ from the slow-forming strains by their mucoid character or their swarming motility or their synthesis of rhamnose. They showed higher swimming mobility.ConclusionsOur results show that the BioFilm Ring Test® is a method specially suited for the study of the initial phase of the formation of a biofilm.Significance and impact of studyThe BioFilm Ring Test® is an easy and rapid alternative to the Crystal violet staining and the enumeration methods. |
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Keywords: | Pseudomonas aeruginosa Cystic fibrosis Biofilm BioFilm Ring Test® |
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