Geographic population structure and sequence divergence in the mitochondrial DNA control region of the Japanese wild boar (Sus scrofa leucomystax), with reference to those of domestic pigs |
| |
| Authors: | Naohiko Okumura Naotaka Ishiguro Masuo Nakano Katsuya Hirai Akira Matsui Makoto Sahara |
| |
| Affiliation: | (1) Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, 080 Hokkaido, Japan;(2) Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, 080 Hokkaido, Japan;(3) Department of Veterinary Microbiology, Gifu University, Yanagido 1-1, 501-11 Gifu, Japan;(4) Nara National Cultural Properties Research Institute, Nijyo-cho, Nara City, 630 Nara, Japan;(5) National Museum of Japanese History, Jyonai 117, 285 Sakura, Japan |
| |
| Abstract: | Mitochondrial DNA (mtDNA) control regions from 40 Japanese wild boars were examined by direct sequencing after amplification by PCR. From the DNA sequences obtained, we found eight haplotypes, whose differences arose via transitions. The geographical distribution of these different haplotypes indicated that wild boar populations inhabited limited areas and that there was some restricted gene flow between local populations. Eight mtDNA haplotypes from Eastern and Western domestic pigs and the Ryukyu wild boar were also analyzed as references to those from Japanese wild boars. The cluster analyses of the control-region sequences showed that those from Japanese wild boards belong to the Asian type as do those from Eastern domestic pigs and the Ryukyu wild boar, which differed from the European type (Western domestic pigs). |
| |
| Keywords: | Japanese wild boar mitochondrial DNA control region domestic pig polymerase chain reaction phylogenetic analyses bootstrap probability |
| 本文献已被 SpringerLink 等数据库收录！ |
|
