| 人工栽培蛹虫草退化现象的分子分析* |
| |
| 引用本文： | 李美娜 吴谢军 李春燕 冯伯森 李庆伟. 人工栽培蛹虫草退化现象的分子分析*[J]. 菌物学报, 2003, 22(2): 277-282 |
| |
| 作者姓名： | 李美娜 吴谢军 李春燕 冯伯森 李庆伟 |
| |
| 作者单位： | 1. 辽宁师范大学生命科学学院,大连,116029
2. 大连双天新药研究开发中心,大连,116031 |
| |
| 基金项目： | 大连市科技局重点推广项目:(2002—05) |
| |
| 摘 要： | 利用PCR-RFLP和RAPD方法对野生驯化蛹虫草及其退化菌种进行了基因水平的分析。PCR-RFLP实验,采用真菌通用引物ITS1和ITS4,扩增出5.8S和其两端的两个转录间隔区(ITS),选用5种识别四个碱基的内切酶(HaeIII、AfaI、TagI、AluI和XspI),其中只XspI酶切结果在两个菌种中存在差异,进一步测序表明,整个片段全长534bp,共有13个位点发生碱基突变,且都为c转换为t。RAPD实验,选用4组计80个随机引物进行PCR扩增,共筛选出9个对所有供试个体均存在明显差异的引物。结果表明了退化菌株较野生驯化菌株在DNA水平上发生了频率较高的突变,这些突变可能直接与导致菌种退化的基因相关联。
|
| 关 键 词： | 驯化菌株 退化菌株 5.8SrRNA RFLP RAPD |
| 文章编号： | 1007-3515(2003)02-0277-0282 |
| 修稿时间： | 2002-06-10 |
MOLECULAR ANALYSIS OF DEGENERATION OF ARTIFICIAL PLANTED CORDYCEPS MILITARIS |
| |
| LIMei-NaWuXie-JunLiChun-YanFengBo-SenLIQing-Wei. MOLECULAR ANALYSIS OF DEGENERATION OF ARTIFICIAL PLANTED CORDYCEPS MILITARIS[J]. Mycosystema, 2003, 22(2): 277-282 |
| |
| Authors: | LIMei-NaWuXie-JunLiChun-YanFengBo-SenLIQing-Wei |
| |
| Affiliation: | LIMei-Na1WuXie-Jun2LiChun-Yan2FengBo-Sen1LIQing-Wei1 |
| |
| Abstract: | PCR-RFLP and RAPD, were used to study the level of difference in gene of naturalized Cordyceps militaris and its degenerative strain.By using PCR-RFLP, 5.8S and its two ITS region were amplified and digested with five restriction enzymes (HaeⅢ、AfaⅠ、TagⅠ、AluⅠand XspⅠ).The sequencing results showed the length of 5.8S and ITS region is 534bp and there are 13 mutant bases. Of 80 primers,9 ones generated reproducible RAPD profiles. The results showed the degenerative strain gene rated high frequency mutation . The mutation may associate with the gene which resulted in degeneration of the strain . |
| |
| Keywords: | naturalized strain degenerative strain 5.8S rRNA RFLP RAPD |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《菌物学报》浏览原始摘要信息 |
|
点击此处可从《菌物学报》下载全文 |
